Micro-photography (10) drugs research contract
Micro-photography (10)- clinical research blog
(6) adjusted depending on diaphragm contraction in field aperture, the condenser to reach the peak increase after the loading station to close down until you see the polygon as the far-field aperture, rotation adjustment screw will depend on the field in aperture the middle notes, and then field aperture zoom into view as the polygon edges cut the outer circle. contract research organization
contract research organization
(7) remove the eyepiece adjustment aperture placed auxiliary telescope, rotating auxiliary telescope to see like a date aperture. Adjustment on the screw, so that aperture in the middle. Large aperture to open to 60% ~ 80%. Tags can also scale computing, such as the use of objective lens 10 Ã-, the numerical aperture (NA) of 0.25, or 0.25 Ã- 80% = 0.2, about aperture of 0.2 on the tone in the scale. The case of objective lens 40 Ã-, NA 0.65, or 0.65 Ã- 80% = 0.52, namely, emphasis on the scale at 0.52. CROs
(8) into the camera eyepiece configured camera eyepiece choice (eyepiece on “PHOTO”) multiples of 10 Ã-. medical social network
(9) selection of optional flat lens achromatic objective lens (N PLAN, PLAN), fluorite objective lens (half-Ping Yang Apochromatic PL FLUOTAR) lens or a flat-field apochromatic objective lens (PLAPO), but the latter is best
(10) pairs of line with the tone adjusted photographs, specimens of the focus of the eye, while observation viewfinder tube, side adjustment on the viewfinder barrel helix, until the “+” character between the separation of the two-lane two-lane until very clear. medical community
(11) shooting in order to “24 Ã- 36″ box, select the parts of the organization as a shooting range.
(12) into film photography device to open the camera back, insert the film in sequence, the left hand by pressing film title sequence into the closed-axis, so that film involved in the revenue piece shaft, after steadily closing the rear cover and wait for 2 ~ 3 seconds species later, heard the sound of motor rotation gear, it will film title sequence into the film axis Raozhuan income housing, some pull-off film on the 24 perforations (3 screen length), the camera on the front window frame count that is presented a “01″ numbers. pharmaceutical research community
(13) put filter with black and white panchromatic film making in general H. E stained green organizations film filter (lamp voltage tends to be used 6 ~ 9V); daylight-type color film with the DLF filters (lamp voltage should be adjusted in the 10.5V and above); light-type color film without filters, but only the lamp voltage is 10.5V or more in tune.
(14) check in the check before exposure “+” word pairs of straight lines, shooting range, the focus on the specimen, voltage number, ASA (film sensitivity), CAL (exposure index), AUTO (auto exposure mode), FIX (hand controlled exposure mode), check and correct before shooting. LEICA MPS 30/60 automatic exposure control, after booting the default way to identify the film’s own sensitivity, CAL 1.00, exposure mode AUTO. CRO
(15) Exposure by LEICA MPS 30/60 automatic exposure control of the “EXPO” button, side buttons, side-axis observation of the camera for the film is rung, the frame number of the window show a increasing numbers, if any ring and show increasing The figures indicated that automatic exposure control in normal operation, later, to continue shooting 2 ~ 36, that is, according to step 12 to 16 steps routinely. If there is no ring or not increasing the number, that is failure. A good film should be recorded for each specimen name, shot content, and magnification and so on. pharmaceutical social network
(16), after finishing the film rewind, the camera will automatically rewind up to full volume emptied. (Such as a roll of film just does not shoot more than a dozen Zhang needs to be filmed part of the film taken out, when according to the camera on the front of the rewind button to rewind the black, recording the number of sheets then shoot for the next and then shot the remaining film), when a title sequence to the sound before they can open the camera back, remove the film. Photo End film to use the black box of paper or film is good, to be washed. Film shoot should be indicated on the box contents, the date finished .Serono Canada
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